Chat Questions and Comments: Masterclass 2
We hope you enjoyed the Masterclass and enjoy browsing the comments and questions that came through the chat during the session. We hope you made some good connections and will join us next week to continue the great discussions.
| 13:31:36 | From Kathy Kahn : 10.30pm in Canberra! Welcome to the late night Australian colleagues |
| 13:31:55 | From Kathy Dibley : thanks Kathy! |
| 13:32:18 | From Jose Barrero : No worries! |
| 13:33:57 | From Hewan Degu : Thank you for making us valuable in this journey. |
| 13:45:12 | From joel hague : I met Angelyne Abuor, she is in Kenya working on cassava breeding |
| 13:45:13 | From Veerendra Sharma : I met Dyg she is involved with Plam transformation. |
| 13:45:18 | From Uchenna Okoroafor : Hi, Everyone. |
| 13:45:20 | From ihuomaokwuonu : I met with dog from malasia |
| 13:45:22 | From Penny Hundleby -JIC : I met Jubilee Park a Grad Student from Uni of Georgia - it was early morning for her at 8.30am. we had a lovely chat. |
| 13:45:23 | From Beata Orman-Ligeza : Pathmasiri, PhD student interested in rice molecular breeding |
| 13:45:25 | From Paul South : I met Amber van Seters from the Netherlands |
| 13:45:28 | From McEnany, Michelle : Wendy Harwood, UK, Works in transformation of cereal crops. |
| 13:45:32 | From III phone : Kiona who is a Grad Student in St. Louis working on gene editing in Cassava |
| 13:45:33 | From Alice Robijns : I spoke to Hamadou from Burkina Faso who is a plant breeder. He is hoping to meet other colleagues who are involved in these kinds of techniques for potential collaboration and learning from each other :D |
| 13:45:34 | From marian quain : Christian, PhD student in Germany working on Wheat and Barley. |
| 13:45:38 | From Abebaw Misganaw Ambaw : I meet a guy from Nederland working his post doc on ENSA |
| 13:45:39 | From Nigel Taylor : Gozda at UC Davis. She has been working on nanoparticle and other non-DNA delivery systems. |
| 13:45:40 | From Matthew Milner : I meet Larissa from INARI she works on soybean transformation and is looking for a way to improve efficiencies. |
| 13:45:40 | From Peggy Ozias-Akins : Leena Tripathi - IITA, currently in Tanzania, main current interest is disease resistant banana |
| 13:45:40 | From Alejandro Perdomo : I met Easter from Kenya an dshe is a Research fellow |
| 13:45:42 | From Kan Wang : I met Barno Rezaeva, from Jochen Kumlen's lab from Germany, she is working on Camelina transformation and engineering |
| 13:45:42 | From Leena Tripathi : I met Peggy Ozias-Akins from USA. She leads peanut improvement |
| 13:45:43 | From Joyce Van Eck : I met Amber van Seters an assistant professor from Wageningen. She is interested in learning the latest in gene editing technologies and getting a better understanding. |
| 13:45:43 | From ihuomaokwuonu : I also met with veranda from Kansas City university |
| 13:45:45 | From Qiudeng Que : I met Minjeong Kang from ISU, graduate student |
| 13:45:45 | From Theodore Moll : I met Laura from the UK. They were interested in learning more about technologies. |
| 13:45:47 | From Wolfgang Zierer : I have met Diaa El-Din Daghma. He is at the IPK Gatersleben and works in Plant Biotechnology, Transformation and Genome Editing. He is interested in learning about new Transformation/GE developments. |
| 13:45:49 | From anindya kundu : I meet Eudald from Georgia working on switchgrass |
| 13:45:51 | From masani : Meet someone from nigeria |
| 13:45:54 | From Gözde Demirer (she/her) : I met Nigel Taylor from Danforth Plant Science Center. He is hoping to learn about technologies and meet people. |
| 13:45:55 | From Benny Ordonez : I met Bahariah from Malaysian, who works on oil palm |
| 13:45:56 | From David Somers : Anna Vittoria Carlucci and I had a nice visit. She is working on cassava. Interested in learning about editing and breakthroughs in cell biology |
| 13:45:56 | From Casandra Schenk : I met Junli (UC Davis) he’s working with wheat and he’s interested to learn new techniques . Very nice talk :) |
| 13:45:56 | From Sung Ryul Kim (IRRI) : Titis from Netherland, she works on casava |
| 13:45:56 | From Jubilee Park : I met Penny Hundleby from the John Innes Center- she works with Brassica transformation. It was lovely to meet her! |
| 13:45:58 | From Gladys Bathan : I met Natalja. She's from Germany working for a seed company. |
| 13:45:58 | From Kaylie Austin : I met with Iris Koeppel PhD student from Germany |
| 13:45:58 | From TJ Higgins : I met Mercy Azanu who is editing grasspea at Iowa |
| 13:45:59 | From Simon Bull : Rahne McIntire: KWS in the USA. Crop development incl sunflowers. |
| 13:45:59 | From Irene M. Fontana : Even if the connection was not really working, I met Amanda! She is a fresh PI and she works in the UK, on photosynthesis :D |
| 13:46:00 | From Kiona Elliott : I met Julius, a research scientist in Uganda. They want to know more about advances in science + gene editing |
| 13:46:01 | From Carl Bernacchi : Satish Viswanathan and I had a great conversation. He is a first year Ph.D. student in Cambridge and knows that plant transformations will be an important part of his career, thus he is taking these online courses. |
| 13:46:02 | From Angel Del Valle Echevarria : I had the pleasure to meet Behailu (KWS-USA) he hopes to learn about new problem solving methods in genome engineering (mainly in transformation/regeneration) |
| 13:46:02 | From Ricky Milne : Aiiphey from Nigeria is keen to get a better understanding on tge basics of transformation |
| 13:46:02 | From Amber van Seters : I met Joce van Elk and Paul South, both were assistant professors in America. Joyce was from a university in New York State and Paul form Louisiana state university. |
| 13:46:02 | From Natasha Yelina : Hi Everyone, I met Daniel who is working on cassava in Ghana. |
| 13:46:03 | From Inez Loedin : Nice to discussed with JiXiang who works in Germany in KWS on sugar beet, wheat and corn plant regeneration. |
| 13:46:03 | From Natalja Beying : I met Gladys from the Philippines. She just started her PhD in molecular technology and plant biotech. Good luck with your PhD! |
| 13:46:03 | From masani : problem with mic |
| 13:46:03 | From Lorena : I enjoyed a lovely conversation with Kensub Lee, a Research Scientist at Iowa State, working on improvement and development of new GE tools. He is looking forward to exposure to current tools used by the rest of the community. He is from South Korea. |
| 13:46:07 | From Kathy Kahn : Franck Ditengou (U Freiburg and from Gabon) works on nitrogen symbiosis/infection and does a lot in Arabidopsis, Medicago, Lotus, barley and he's interested in what's happening in other crops. Also we are both curious about how to bridge more effectively with colleagues in Africa! |
| 13:46:07 | From Nicholas Ferrari : I met with Temitope for a great chat! |
| 13:46:08 | From Christian Hertig : I met Marian Quain from Ghana working with sweet potato and soybean |
| 13:46:10 | From Todd : Hi MJ! |
| 13:46:11 | From Behailu Aklilu : Angel Del Valle Echevarria From Switzerland. He is currently a consultant for NGO involved in agriculture located in Hawaii. He is interested to gain information on new plant transformation and tissue culture technologies that can be applied to genome editing. |
| 13:46:12 | From Titis Wardhani : I met Sung Ryul Kim from Philippines. He works at IRRI and is currently busy with the rice transformation and gene editing. We discussed a lot about transformation. It is nice to meet you, Kim! |
| 13:46:15 | From Junli Zhang : I met Casandra. She is from Mexico and now works in Germany. She studies Tomato root and has a lovely son! |
| 13:46:15 | From Pathmasiri Karunarathne : I met Emma Wallington from Cambridge, Beata Orman from Cambridge an Jen Pietrre fro Brazil. |
| 13:46:16 | From Ally Weir : I met Sarah Garland, she is a post-doc in science policy and is interested in learning more about editing for the developing world |
| 13:46:16 | From GD43514 : I met Sebastian who is Cambridge close to where I am based currently (Dundee) |
| 13:46:16 | From Sung Ryul Kim (IRRI) : good to see you, Titis |
| 13:46:21 | From Beata Orman-Ligeza : Jean Pierre PhD studen from Liege, with an interest in cassava breeding looking to learn CRISPR |
| 13:46:22 | From Emma Wallington : I met Pathmasiri from IRRI & Jean Pierre from Liege who are both PhD students hoping to get tips for the future. And Beata fromCambs |
| 13:46:23 | From Wendy Harwood : Great chat with Michelle McEnany who is with Corteva focusing mainly on field work but keen to learn the transformation part. |
| 13:46:24 | From Indieka Abwao : I met Jason Zurn, from the USAPost doc working on wheat and he is interested in learning more about CRISPR |
| 13:46:24 | From Mauricio Grisolía : I've met David Nelso, from California. Research assistant from the UC. Intrested in somke signal pathway |
| 13:46:25 | From Pingdewinde Adelaide Ouedraogo : I met ANTOINE from Ruanda a University lecturer and Uchenna fri Nigeria working on Cassava. |
| 13:46:26 | From Amanda Cavanagh : I met Irene Fontana, she is in Germany researching barley drought responses and strigolactones! |
| 13:46:26 | From Rik Huisman : I met Abebaw. He Is located in Ethiopia, and is interested to learn how large numbers of genes can be succesfully transferred to crops at once. |
| 13:46:27 | From MZaporteza : hello I et Jason from the UK and he is into vertical aeroponics, and his interest in gene editing is to develop crops for the aeroponics set up |
| 13:46:29 | From Jeffrey Staub : I met Federico and Chantal, student in Cambridge and Rwanda |
| 13:46:29 | From Sophia Müller : Diana from Rwanda is a molecular biologist who is working as a Research fellow in an agricultural Institution studying crops like cassave, banana and coffee! She is very interested in plant Transformation and Genome editing in general |
| 13:46:30 | From Christina Krönauer : I met Kathy Dibley from Canberra |
| 13:46:37 | From Rabih Mehdi : I met Digna Swazi from Tanzania and she is working as a Associate Breeder with Cucumber and Pumpkin and she is looking forward to learn new technologies on genome editing and also hopes to use it in her PhD |
| 13:46:38 | From Sarah Garland : I met Ally Weir who is doing a PhD in California working with lettuce |
| 13:46:38 | From Federico Marangelli : I met Jeffrey Staub from USA working with soybean and corn and Chantala (PhD student) working with cassava |
| 13:46:45 | From Lene Heegaard Madsen : I met Rose from Nairobi working on Cassava and Benedetta from GB working in ENSA |
| 13:46:50 | From Walter Dauksher : I met Rahne and Simon. Rahne works at kus and Simon works at eth. It was a pleasure to meet both! |
| 13:46:50 | From Juan Pablo Arciniegas Vega : Kimberly Nelson. She works with genetic transformation and gene editing in sorghum. She is looking for new ideas and perspectives on these technologies. |
| 13:46:50 | From Beata Orman-Ligeza : Emma Wallington, PI at NIAB leading Crop transformation team |
| 13:46:55 | From Keunsub Lee : I met Lorena Moeller from Guatemala, USA. She works for Benson Hill and work as a director of trait delivery and is interested in new technologies. |
| 13:46:57 | From Mei : I met with Isaac from Kenya, a postdoc working on GE in grass pea. He is looking for improving transformation in difficult speices. |
| 13:46:58 | From Dr. Aiiphey : I met are Richie Milne, he works in CSIR Australia and works in a lab working to develop disease resistance in sorghum and rice |
| 13:47:01 | From Erwin Arcillas (IRRI) : I met Joseph, a grad student from US and he is interested in the application of genome-editing in plants |
| 13:47:03 | From Ousmane Boukar : I met Eleni that is working with Rogers as crop. Center manager and mostly on Barley. |
| 13:47:03 | From Satish Bharathwaj Viswanathan : I had a interesting chat with Carl Bernacchi from University of Illinois. |
| 13:47:10 | From Bahariah : I met Benny Ordonez she doing PHD on potato CRISPR genome editing in Univ. of California, Davis, USA. |
| 13:47:10 | From Dr. Daniel Dzidzienyo : Met Natasha, a postdoc at the University of Cambridge |
| 13:47:13 | From Christian Lamm : Aleksandr Gavrin, Geneticist working on root nodules, especially interested in CRISPR |
| 13:47:15 | From Mercy K Azanu : I met Dr. TJ Higgins, who is based in Australia and working on Bt Cowpea in Burkina Faso, Ghana and Nigeria |
| 13:47:20 | From Uchenna Okoroafor : I met with Adelaide(Burkinafaso), a Plant breeder working on Cowpea; and Antoine (Rwanda) a University lecturer …Biology/Crop Science |
| 13:47:20 | From Hamadou : good to seen you Alice |
| 13:47:22 | From Soumaya Zaidi : I met two researchers from UK, they are working on wheat transformation and editing in order to increase yield, they are working also on male sterility and several other topics. |
| 13:47:24 | From Chantal : I met Jeffrey from USA, he will our a speaker, working on corn and I met Federico Master student from UK |
| 13:47:26 | From Jean Pierre Bizimana : I met Emma Wallington |
| 13:47:27 | From Jo K : I met Ping Che at Corteva USA, working on philanthropic project, funded by M+B Gates foundation, in sorghum and cow pea transformation. |
| 13:47:31 | From Digna Swai : I met Rahib PhD student in Germany....he working on Potatoes breeding. |
| 13:47:40 | From Iris Koeppel : I met Kaylie Austin from KWS, US, and she is interested in the progress in transformation technologies |
| 13:47:45 | From Julius Pyton Sserumaga : I met Kiona, a grad Student working on gene editing in cassava for disease resistance |
| 13:47:58 | From Eudald Illa-Berenguer : Hi everyone, I met Anindya Kundu from Cambridge. He works in Fragaria - both diploid and octoploid cultivars - |
| 13:48:11 | From Richard Garcia : I met Kate Creasey from New York interested in plant transformation for rapid growth system especially for their growth chambers for various crops |
| 13:48:26 | From Diana(RAB) : I met Sophia, pursuing PhD at Wageningen, doing research on nitrogen |
| 13:48:45 | From Kathy Dibley : I enjoyed meeting with Christina Kronauer, a postdoc from Germany now at Aarhus working with Jens Stougaard on nitrogen symbiosis recognition signalling. She is here to learn and update her knowledge in this area. |
| 13:48:49 | From rezaeva : I met with Kan Wang from Iowa State University who is one of the organizers in the masterclass. Her aim is to teach researchers especially those whose people as young scientists |
| 13:49:12 | From Temitope Jekayinoluwa : I met Nicholas a first year PhD candidate from Louisiana State University. Nicholas is passionate about his work and interested on how to generate robust transformants |
| 13:49:47 | From Sultan Alhusayni : I met with Vincent a graduate student who is working on turf grass genome editing form Georgia USA. |
| 13:49:49 | From dyg : hai.. i met ihuomao frm nigeria n veerendra from india |
| 13:50:40 | From Afeez Oluniyi Shittu (IRRI) : I met Samuel from IITA-Nairobi. |
| 13:50:57 | From Mediatrice : I am Mediatrice , from Rwanda üá∑üẠ|
| 13:51:05 | From Franck Ditengou : I had a chat with Kathy Kahn (UK), she acts a Gates foundation coordinator for the ENSA project. She is interested in building bridges with colleagues in Africa. She is also trying to see how to connect the different projects, and help the development of the community |
| 13:57:31 | From Indieka Abwao : Question? How do you make protoplast to take up DNA/Construct?/gene of interests? are there any special construct designed for transforming protoplast? |
| 13:58:06 | From Antoine Nsabimana : Hi! I met Pingdewing from Burkina Faso and she is at INERA and met also Uchema from Nigeria, she is working on Cassave at Research Institute. |
| 13:58:24 | From Behailu Aklilu : Questions : When there is genotype dependency for a given species of crop plant for callus induction or regeneration, which media component do you usually start to try to optimize first? Hormone ? or others ? |
| 13:59:27 | From Rahne McIntire : I met Simon who works on Cassava in Switzerland. I also met Walt who is a new PhD student in Georgia new to tissue culture. |
| 14:01:24 | From Behailu Aklilu : Good recycling of water bottle !! |
| 14:03:08 | From Ikechukwu Nnaji : I met Tomas Cermak, a plant geneticist,who works in Boston. Primarily interested in gene transfer of useful traits from wild species to the domesticated crops. |
| 14:06:34 | From Abigiya Germame : Thank you for the nice presentation |
| 14:07:18 | From Jean Pierre Bizimana : Thank you Joice for a nice presentation |
| 14:07:29 | From dyg : tq...nicely shared |
| 14:07:33 | From Christian Rogers : Questions for Joyce in the chat please… |
| 14:07:43 | From Nigel Taylor : What controls do you run with your genes of interest to assess for potential problems in an experiment. |
| 14:07:44 | From Todd : Excellent talk, Joyce! Agree with all of your ‚Äúwords of wisdom‚Äù. üëç |
| 14:07:49 | From dyg : have u tried RFP? |
| 14:07:50 | From Franck Ditengou : Do you have any idea why some tissues regenerate and some not? Why is so different from plant to plant |
| 14:07:55 | From Kacper Bonter : Great talk and advice! Could you say a few words on how you’re working with bryophyte transformation (hornworts)? Do you use hormones like with angiosperms during regeneration? |
| 14:08:04 | From David Somers : Thoughts on transformation of elite genotypes? |
| 14:08:14 | From Wolfgang Zierer : Have you aquired any insight over the years on why some genotypes are so much more amendable to Transformation than others? |
| 14:08:28 | From Benta Sina : Thank you for your interesting presention. |
| 14:08:49 | From David C Nelson : When you use visible markers like GFP for selection, do you also apply an herbicide selection to reduce growth of non-transformed cells in the same callus/explant? Or do you just excise GFP-positive tissues as they grow? |
| 14:08:52 | From Nicholas Ferrari : When screening accessions for transformation, are there common traits that you seek out or avoid in pursuit of high efficiency? |
| 14:08:53 | From ihuomaokwuonu : Questions: At what stage do actually using the plastic bottles for weaning and what substrate do you use |
| 14:08:53 | From marian quain : With your regeneration protocols, which system work better for transformation? Direct or indirect organogenesis? |
| 14:09:23 | From Hewan Degu : Joyce, can you just highlight the needed laboratory facility to run TC? |
| 14:09:46 | From Diaa El-Din Daghma : thanks for the nice brief introduction, but what about the quality of plant donor material and its growth conditions optimization |
| 14:10:32 | From Mauricio Grisolía : have you tried gene gun for genome edition? |
| 14:10:48 | From Junli Zhang : Very nice talk. Thank you. Question, after regeneration, if can not form root, how do you solve that problem?Thanks |
| 14:10:58 | From dyg : tq 4d answer |
| 14:11:31 | From Rabih Mehdi : Thank you for the nice talk! Do you see a difference when using different Agrobacteria strains? |
| 14:12:04 | From Soumaya Zaidi : what makes the plant regenerate from the hypocotyl and not from cotyledons? |
| 14:12:21 | From Kacper Bonter : thank you so much for answering! |
| 14:13:33 | From Titis Wardhani : Hi, thank you for the talk! Could you elaborate more about the choice of gelling agent? How does that make difference in transformation efficiency? Thanks! |
| 14:20:53 | From David Somers : Thank goodness Bill was inspired by the microinjection work in maize |
| 14:25:39 | From Todd : Thank you, Dave, for inspiring Bill to go in a different direction! |
| 14:27:03 | From Jacob : Are you using the maize BBM and WUS in wheat and sorghum or did you find the homologs? |
| 14:27:21 | From Juan Pablo Fernandez : Did you try any other "quick" system, like GRF-GIF? Is there any prefference from WUS/BBM over other system? |
| 14:29:31 | From Jose Barrero : Are those methods working well in divots? Thanks |
| 14:30:32 | From Christian Hertig : Thanks for your talk and all Information you shared with us. Do you think, early tissue-specific expression of only BBM might give also successful increase of regeneration efficiency to finally improve transformation efficiency? |
| 14:40:11 | From joel hague : HI Bill, you referred to excision of the DRs...same method (Cre/lox) as the original paper (2016)? |
| 14:40:36 | From Wolfgang Zierer : What happens in dicots using the WUS/BBM System? |
| 14:40:44 | From Christian Rogers : Questions for Bill please? |
| 14:40:47 | From Ally Weir : Hi, can you clarify what you mean by “drop out” from the last few slides> |
| 14:40:47 | From David Somers : Any luck with delivering just the Bbm Was proteins? |
| 14:40:48 | From anindya kundu : Can altruistic method lead to increase the size of gene delivery which usually is very low with conventional methods |
| 14:40:59 | From masani : Why stop working with microinjection? Have you tried your BBM/Wus system with difficult plants such as rubber |
| 14:41:00 | From Nigel Taylor : Does the Baby Boom Wuschel technology transfer to dicots? |
| 14:41:49 | From Leena Tripathi : Whether anyone at Corteva has tried using Wus/Bbm in vegetatively propagated crops? |
| 14:42:07 | From Natasha Yelina : Wonderful talk, thank you. Have you ever tried to increase plant susceptibility to Agrobacterium virulence by knocking out PAMP receptor - similar to efr in Arabidopsis? Thank you |
| 14:42:11 | From Wendy Harwood : How do you think WUS2 alone stimulates editing efficiency? |
| 14:42:15 | From Titis Wardhani : Hi Bill, thank you for the talk! Did you use the endogenous BBM/WUS orthologous in sorghum? Or, did you introduce the maize BBM.WUS in sorghum? Thanks |
| 14:42:32 | From Inez Loedin : Bill, did you do the WUS for HDR to mainly do insertion in targeted locus or also for allele replacement? How was the frequency for allele replacement? |
| 14:44:21 | From Christian Hertig : You used maize WUS2 and BBM for spring wheat. Have you investigated if wheat orthologs of WUS and BBM can also be used to improve wheat Regeneration/Transformation? |
| 14:45:34 | From Rabih Mehdi : Is it necessary to get rid of the WUS booster when using it in combination with a CRISPR/Cas system? Are there any developmental issues when WUS is active constitutively? |
| 15:05:29 | From Jose Barrero : Do you know if the GRF genes also help in direct regeneration systems (without callus)? |
| 15:07:01 | From Minjeong Kang : What is the phenotype of the GRF overexpressed plants? If the GRF expressing plants looks normal like wild type, is there any changes in cellular level? |
| 15:09:33 | From Simon Peter : From experience, what is the best method to introgress transformable gene into recalcitrant genome for easy genetic transformation. |
| 15:16:31 | From Christian Rogers : Cameras on for a quick pairs discussion: What did you take from David’s talk? |
| 15:17:18 | From ihuomaokwuonu : Can we translate this technology into product pipeline |
| 15:17:37 | From Rahne McIntire : How is transformation efficiency measured? |
| 15:17:38 | From Nigel Taylor : Why do you think the A. thaliana GFP works better in beet the the native gene? |
| 15:18:10 | From Hewan Degu : why promoter selection is important in the transformation. |
| 15:18:39 | From Qiudeng Que : For organogenic system, does GRF5 enhanced transformation reduce chimera rate? |
| 15:18:43 | From Wolfgang Zierer : Have you also played arround with chemical boosters? |
| 15:18:48 | From Monica Prias : Thanks David for a very nice presentation, How did you evaluate the plants to conclude that overexpression of GRF does not affect their development? |
| 15:18:55 | From Christian Lamm : Did you try skipping the callus stage and go for direct transformation of eg leaf explants |
| 15:19:54 | From Titis Wardhani : Hi David, thanks for the talk. How did you choose the GRF gene to work on? As the GRF has another copies in their gene family. |
| 15:20:08 | From Abebaw Misganaw Ambaw : why did you interrogate two promotors during gene model construction and vector preparation? |
| 15:20:55 | From Huijun Liu : Do you tried GRF-GIF constructs? |
| 15:25:02 | From Christian Rogers: JUANs questions started here |
| 15:46:27 | From David Pacheco : ZmGRF1 that I showed is the one published by Dirk Inze (VIB, Belgium). @Juan D. Bernardi: I do not know whether that is the ortholog of wheat GRF1. |
| 15:53:08 | From Veerendra Sharma : That's a very good talk. My question is do you think if we use GRF-GIF from the same species for transformation would produce better results? |
| 15:55:12 | From vveena : what are the different agrobacterium strains you have tried, is one strain better than other or it does not matter? |
| 15:59:18 | From kammwj : Great answer - I agree |
| 16:01:07 | From Kiona Elliott : Thanks for the nice talk @Juan. Do you have any idea why there is no phenotype difference in wheat but there is in the dicots at later development stages? Could the chimera possibly be silenced in wheat at later stages, but not in dicots? |
| 16:04:15 | From Juan : Thanks Kiona. Actually there are phenotypic differences, GRF-GIF plants produce a little larger leaves and grains. What I was trying to say is that they do not have phenotypes that affect fertility or normal development. |
| 16:05:22 | From Kiona Elliott : Okay, thank you! |
| 16:19:46 | From Christian Rogers : Questions for Qiudeng in the chat please…. |
| 16:21:55 | From laurens.pauwels@ugent.vib.be : Very interesting technology! What is your estimate of the efficiency of HI-Edit (edited plants/haploids obtained)? |
| 16:22:30 | From David Somers : Great talk, you mentioned that there were variable results with different maize heterotic groups. Could you elaborate on this effect or reasons for it |
| 16:22:45 | From Simon Peter : Do you have maternal effect during the crosses |
| 16:24:00 | From Simon Peter : Thanks for the talk |
| 16:24:20 | From David Somers : Any luck with homologous recombination insert edits? |
| 16:26:56 | From David Somers : Thanks Qiudeng |
| 16:52:45 | From Lorena : Any experience on transient approaches to effect epigenetic modifications? |
| 16:53:22 | From Juan : have you check off-targets effects of the epiallele technology? |
| 16:58:11 | From Lorena : Thank you for a great session! |
| 16:58:19 | From Ally Weir : Thank you all. |
| 16:58:20 | From anna vittoria carluccio : Thank you |
| 17:11:21 | From Ihuoma : what would be the expected regulatory perspective to these new technologies |
| 17:16:31 | From Nikolai : how can new technology target plastids within plant cells with greater accuracy? |
| 17:19:29 | From Jauhar Ali : Thanks to all the speakers for their excellent talks |
| 17:21:04 | From joel hague : Thank you!!!! |
| 17:24:28 | From MZaporteza : thanks to all the speakers! I really appreciate all the talks, very helpful to explore non model and recalcitrant crops |
| 17:35:02 | From Samwel Muiruri Kariuki : Has anyone tried BBM/wus or grf-gif systems by silencing their repressors? |
| 17:35:21 | From US NSF - Diane Okamuro : Thanks so much to all the speakers! It is so important for all to understand where the leading edge is for the field of plant transformation and gene editing. Thanks also to the organizers for putting together such an impressive group of speakers! Well done! |
| 17:38:40 | From Hewan Degu : Thank you. |
| 17:39:05 | From Rabih Mehdi : Thank you very much for the fantastic Masterclass and the great talks!! |
| 17:39:14 | From Indieka Abwao : Thank you |