Workshop: Principles and Best Practices of Genome Engineering

This session was presented at the 2023 In Vitro Biology Meeting held in Norfolk, Virginia from June 10 - 14, 2023 and was recorded as part of the In-Person and On-Demand Limited Access Program. This session was divided into two parts:

Part 1: Despite its usefulness, genome editing is not always easy to implement, especially when there are crop-specific considerations. A critical part of genome engineering is first mastering the methods for plant tissue culture and transformation. Therefore, the first half of this workshop covers the best practices for these critical steps. We address how to increase success for working with established systems and how to develop systems for new species. The PlantGENE Steering Committee will lead the discussion, with workshop attendees being strongly encouraged to share their knowledge. Prior to the workshop, a survey was sent to solicit ideas for discussion items that are of the greatest interest to attendees.

Part 2: (Beginning at time stamp 2:04:00) The second half focuses on using state-of-the-art CRISPR genome engineering tools in plants; including design, construction, and application of multiplexed CRISPR-Cas9, base editing, and the CRISPR-Combo system. These presentations cover the background and principles of the technologies, with details provided on vector design and construction, optional assessment in protoplasts, and analysis of transformed protoplasts and stable lines for identification of edited events. All the vectors, analysis tools, and data sets used in the workshop are publicly available.